کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090387 1081499 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sterilization of biological pathogens using supercritical fluid carbon dioxide containing water and hydrogen peroxide
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Sterilization of biological pathogens using supercritical fluid carbon dioxide containing water and hydrogen peroxide
چکیده انگلیسی

Novel noninvasive techniques for the removal of biological contaminants to generate clean or sterile materials are in demand by the medical, pharmaceutical and food industries. The sterilization method described here uses supercritical fluid carbon dioxide (SF-CO2) containing 3.3% water and 0.1% hydrogen peroxide (v/v/v) to achieve from four to eight log viability reduction of all tested microbial species, including vegetative cells, spores and biofilms. The sterilization method employs moderate pressure and temperature (80 atm, 50 °C) and a short (30-minute) treatment time. The procedure kills various opportunistic pathogens that often persist in biofilm structures, fungal spores commonly associated with nosocomial infections, and Bacillus pumilus SAFR-032 endospores that are notoriously hard to eradicate by conventional sterilization techniques.


► We optimize SF-CO2–H2O2 sterilization to minimal efficient conditions.
► We model inactivation kinetics for Bacillus pumilus SAFR-032 spores.
► Sterilization is effective for organisms in biofilms, endospores and fungal spores.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 87, Issue 1, October 2011, Pages 70–75
نویسندگان
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