کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2096648 1082175 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of essential physical properties of vitrification solutions
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Measurement of essential physical properties of vitrification solutions
چکیده انگلیسی

Vitrification is an “ice-free” cryopreservation method that has rapidly developed in recent years and might become the method of choice for oocyte cryopreservation. Five sources of damage should be considered when attempting to achieve successful oocyte cryopreservation by vitrification:1.Solution effects2.Crystallization3.Glass fractures4.Devitrification and recrystallization5.Chilling injuryThe probability of successful vitrification depends on three major factors: viscosity of the sample; cooling and warming rates; and sample volume. One of the problems associated with the vitrification solution is that it may contain high concentrations of cryoprotectants (CP), which can damage the cells through chemical toxicity and osmotic shock. In the present study, we examined the principal parameters associated with successful vitrification, and attempted to compose guidelines to the most important aspects of the vitrification process.The first step was the selection of a suitable and least toxic vitrification solution. We then evaluated the effects of cooling rate and volume on the probability of vitrification. Reduction of the sample volume, combined with accelerated cooling, enabled reduction of the CP concentration. However, in practice, a delicate balance must be maintained among all the factors that affect the probability of vitrification in order to prevent crystallization, devitrification, recrystallization, glass fractures and chilling injury.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 67, Issue 1, 1 January 2007, Pages 81–89
نویسندگان
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