کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2097358 1082215 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Endogenously generated hydrogen peroxide induces apoptosis via mitochondrial damage independent of NF-κB and p53 activation in bovine embryos
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Endogenously generated hydrogen peroxide induces apoptosis via mitochondrial damage independent of NF-κB and p53 activation in bovine embryos
چکیده انگلیسی

Hydrogen peroxide (H2O2) has been implicated as a key molecule in arresting embryonic development; however, its mechanism of action is not fully established. The aim of the present study was to determine the chronological generation of H2O2 from oocyte to morula, and to examine the relationship of H2O2 with loss of mitochondrial membrane potential, nuclear factor kappa-B (NF-κB), p53, caspase-3 activation, and cell death in bovine embryos in vitro. Accordingly, superoxide anion radicals were detected between 32 and 120 h after in vitro fertilization, but higher percentages of oxygen radicals were found in non-competent embryos (n = 73, 22 to 34%) than in competent embryos (n = 73, 0 to 1%; P < 0.005). Similarly, H2O2 levels were higher in non-competent embryos (n = 249, 39 to 71%) than in competent embryos (n = 278, 0 to 3.4%) at all developmental stages tested (P < 0.005). The percentage of cells with apoptotic morphology were higher in non-competent embryos (n = 411, 3 to 54%) than in competent embryos (n = 306, 0 to 0.6%; P < 0.005). Based on assessment of mitochondrial membrane potential, competent embryos (n = 305) had the highest percentages of JC-1 staining (31 to 50%) when compared with non-competent embryos (n = 411; 1 to 15%, P < 0.005). The percentage of activation of general caspases was different in non-competent embryos (n = 291, 15 to 57%) when compared to competent embryos (n = 304, 0 to 0.5%; P < 0.005). Pharmacological inhibition of caspase-3, NF-κB and p53 triggered aberrant embryo cytoplasmic fragmentation with and without nuclei. We concluded that the sequential mechanism of O2− and H2O2 generation, mitochondrial damage, caspase activation, and apoptotic morphology might be responsible for the developmental arrest of preimplantation embryos.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 67, Issue 7, 15 April 2007, Pages 1285–1296
نویسندگان
, , , ,