Critical values of the volumetric oxygen transfer coefficient and oxygen concentration that prevent oxygen limitation in ferrous iron and elemental sulfur oxidation by Acidithiobacillus ferrooxidans

• Minimum aeration conditions to exclude O2 limitation were defined for Acidithiobacillus ferrooxidans cells.
• The parameters (kLa)crit and Ccrit were determined for bacterial Fe2 + and S0 oxidation.
• The Ccrit value was validated with a bioreactor suspension of A. ferrooxidans.

Biochemical and biotechnological aspects of aeration conditions in iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans cultures were studied. The critical values of volumetric oxygen transfer coefficient, (kLa)crit, and oxygen concentration, Ccrit, were used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation during substrate oxidation by the model bioleaching culture. The Michaelis constants for oxygen were 1.07 and 0.61 μmol O2 l− 1 for the oxidation of ferrous iron and elemental sulfur, respectively. The Ccrit values, below which oxygen limitation may occur, were 6.250 and 3.125 μmol O2 l− 1, determined in respirometric experiments for the oxidation of ferrous iron and elemental sulfur, respectively. The rates of iron and sulfur oxidation were also determined at the Ccrit values by measuring the formation of ferric iron and protons in the corresponding cell suspensions. Neither iron nor sulfur oxidation was limited by oxygen at Ccrit. The (kLa)crit values required to maintain oxygen-unlimited oxidation of ferrous iron and elemental sulfur were 7.70 and 4.88 h− 1, respectively.