3D Cell-SELEX: Development of RNA aptamers as molecular probes for PC-3 tumor cell line

• 3D cell culture spheroids mimic the tumor microenvironment.
• Aptamers selected in 3D cell culture is ideal for discovery of novel markers.
• Small aptamers with spontaneous folding (−∆G) were enriched against tumor spheroids.
• Aptamers may be used as potential markers for prostate cancer.

Human prostate cancer (PCa)