کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2130165 1086534 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
miR-184 and miR-150 promote renal glomerular mesangial cell aging by targeting Rab1a and Rab31
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
miR-184 and miR-150 promote renal glomerular mesangial cell aging by targeting Rab1a and Rab31
چکیده انگلیسی


• miR-184 and miR-150 were differentially expressed in old rat renal tissues.
• miR-184 and miR-150 were increased significantly in aging glomerular mesangial cells.
• miR-184 and miR-150 suppressed the expression of Rab1a and Rab31.
• miR-184 and miR-150 inhibited autophagy and promoted oxidative damage.

The molecular mechanism of kidney aging is not well understood, but the abnormal expression of miRNAs with aging is considered to be an important contributor. miR-184 and miR-150 were screened using a miRNA microarray and qRT-PCR and found to be significantly upregulated in 24-month-old rats. Rat renal primary glomerular mesangial cells (GMCs) were isolated from 3-month and 24-month-old rats for the in vitro analysis of the roles of miR-184 and miR-150 in kidney aging. Bioinformatics analyses suggested that Rab1a and Rab31, which are associated with cell autophagy, were targeted by both miR-184 and miR-150. miR-184 and miR-150 were increased significantly in aging GMCs versus young cells, while Rab1a and Rab31 were significantly lower in aging cells. Furthermore, dual luciferase reporter assays revealed that miR-184 and miR-150 bound to the 3ʹ-UTR of Rab1a and Rab31 mRNAs. Transfection of miR-184 and miR-150 mimics into young GMCs suppressed the expression of Rab1a and Rab31. Transfected cells showed lower autophagy activities and higher levels of cellular oxidative products, leading to the aging of young GMCs. However, miR-184 and miR-150 inhibitors promoted autophagy and reduced oxidative damage by upregulating Rab1a and Rab31 in old GMCs. In conclusion, miR-184 and miR-150 inhibited autophagy, promoting GMC aging.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 336, Issue 2, 15 August 2015, Pages 192–203
نویسندگان
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