کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2130384 | 1547706 | 2014 | 10 صفحه PDF | دانلود رایگان |

• TREX2 complex member PCID2 but not ENY2 localizes to the centrosome in HeLa cells.
• Centrin 2 is required for the localization of PCID2 at the centrosome.
• PCID2 is found at the centrosome in G1/S at a slightly higher rate than that in G2/M.
• PCID2 but not ENY2 delays the rate of nuclear protein export.
• Co-depletion of centrin 2 and PCID2 leads to blocking rather than delaying nuclear protein export.
TREX-2 is a five protein complex, conserved from yeast to humans, involved in linking mRNA transcription and export. The centrin 2 subunit of TREX-2 is also a component of the centrosome and is additionally involved in a distinctly different process of nuclear protein export. While centrin 2 is a known multifunctional protein, the roles of other human TREX-2 complex proteins other than mRNA export are not known. In this study, we found that human TREX-2 member PCID2 but not ENY2 is involved in some of the same cellular processes as those of centrin 2 apart from the classical TREX-2 function. PCID2 is present at the centrosome in a subset of HeLa cells and this localization is centrin 2 dependent. Furthermore, the presence of PCID2 at the centrosome is prevalent throughout the cell cycle as determined by co-staining with cyclins E, A and B. PCID2 but not ENY2 is also involved in protein export. Surprisingly, siRNA knockdown of PCID2 delayed the rate of nuclear protein export, a mechanism distinct from the effects of centrin 2, which when knocked down inhibits export. Finally we showed that co-depletion of centrin 2 and PCID2 leads to blocking rather than delaying nuclear protein export, indicating the dominance of the centrin 2 phenotype. Together these results represent the first discovery of specific novel functions for PCID2 other than mRNA export and suggest that components of the TREX-2 complex serve alternative shared roles in the regulation of nuclear transport and cell cycle progression.
Journal: Experimental Cell Research - Volume 320, Issue 2, 15 January 2014, Pages 209–218