کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2130661 1086590 2012 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Targeting high affinity and epitope-distinct oligoclonal nanobodies to HER2 over-expressing tumor cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Targeting high affinity and epitope-distinct oligoclonal nanobodies to HER2 over-expressing tumor cells
چکیده انگلیسی

Modern anti-HER2 antibody therapy tends to exploit a panel of different antibodies against different epitopes on the antigen. For this aim, nanobodies are very striking targeting agents and can be easily produced against any cell-specific membrane antigen. The oligoclonal nanobodies can be used to block more than one functional epitope on a target antigen and inhibit the generation of escape variants associated with cancer therapy. In this study, 12 nanobody clones selected from an immune camel library were examined for their ability to differ between tumor markers. These oligoclonal nanobodies targeted breast cancer cells better than each individual nanobody. In epitope mapping, several nanobodies overlapped in the epitope recognized by trastuzumab and some of the non-overlapping nanobodies could affect the binding of trastuzumab to HER2. This study demonstrates that the oligoclonal nanobodies are potential therapeutic tools that can be used instead of, or in combination with trastuzumab to assess tumor viability during treatment.

Figure optionsDownload high-quality image (173 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 318, Issue 10, 10 June 2012, Pages 1112–1124
نویسندگان
, , , , , ,