کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2131079 1086621 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Domain analysis of α-actinin reveals new aspects of its association with F-actin during cytokinesis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Domain analysis of α-actinin reveals new aspects of its association with F-actin during cytokinesis
چکیده انگلیسی

α-actinin is a rod-shaped actin cross-linking protein composed of actin binding domain, spectrin-like repeats of the central rod domain and the EF-hand domain. Cytokinesis in mammalian cells involves remodeling of equatorial actin filaments (F-actin) mediated by α-actinin. However, it remains unknown how α-actinin interacts with F-actin at the cleavage furrow. To address this question, we have conducted functional analysis of the mutant that either lacks the ability to cross-link F-actin (ABD) or to bind to F-actin (ΔABD). We found that equatorial localization of α-actinin requires both its F-actin binding and cross-linking activities. Unexpectedly, we also found that overexpression of ΔABD-GFP but not ABD-GFP frequently caused accelerated cytokinesis and ectopic furrowing similar to those observed in cells depleted of α-actinin. Immunofluorescence revealed that overexpression of ΔABD-GFP caused displacement of endogenous α-actinin and a decrease in the density of F-actin throughout the entire cortex. Biochemical experiments showed that ΔABD was able to form heterodimers with endogenous α-actinin. These results suggest that the central rod spectrin-like repeats of α-actinin is sufficient for its dimerization in vivo. Our findings uncover previously unappreciated functions of the α-actinin domains in a cell.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 316, Issue 12, 15 July 2010, Pages 1925–1934
نویسندگان
, , , , ,