کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2131465 | 1086643 | 2009 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
p53 regulates the proliferation, differentiation and spontaneous transformation of mesenchymal stem cells
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کلمات کلیدی
p53reverse transcription quantitative PCRs.cCFSEhMSCFCSMSCRT-qPCRsubcutaneously - زیر جلدیfoetal calf serum - سرم گوساله جنینTumor stem cells - سلول های بنیادی تومورMesenchymal stem cells - سلول های بنیادی مزانشیمیhuman mesenchymal stem cells - سلول های بنیادی مزانشیمی انسانیTumor cells - سلول های تومورbone marrow - مغز استخوانwild type - نوع وحشیPropidium iodide - پروتئین یدیدcarboxyfluorescein diacetate succinimidyl ester - کربوکسیفلوورسسین دی سکته سوکسینیمیدیل استر
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Mesenchymal stem cells (MSC) have been extensively studied and gained wide popularity due to their therapeutic potential. Spontaneous transformation of MSC, from both human and murine origin, has been reported in many studies. MSC transformation depends on the culture conditions, the origin of the cells and the time on culture; however, the precise biological characteristics involved in this process have not been fully defined yet. In this study, we investigated the role of p53 in the biology and transformation of murine bone marrow (BM)-derived MSC. We demonstrate that the MSC derived from p53KO mice showed an augmented proliferation rate, a shorter doubling time and also morphologic and phenotypic changes, as compared to MSC derived from wild-type animals. Furthermore, the MSC devoid of p53 had an increased number of cells able to generate colonies. In addition, not only proliferation but also MSC differentiation is controlled by p53 since its absence modifies the speed of the process. Moreover, genomic instability, changes in the expression of c-myc and anchorage independent growth were also observed in p53KO MSC. In addition, the absence of p53 implicates the spontaneous transformation of MSC in long-term cultures. Our results reveal that p53 plays a central role in the biology of MSC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 315, Issue 20, 10 December 2009, Pages 3598-3610
Journal: Experimental Cell Research - Volume 315, Issue 20, 10 December 2009, Pages 3598-3610
نویسندگان
Alejandro Armesilla-Diaz, Gema Elvira, Augusto Silva,