کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2131725 1086656 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo inhibition of epidermal growth factor receptor autophosphorylation prevents receptor internalization
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
In vivo inhibition of epidermal growth factor receptor autophosphorylation prevents receptor internalization
چکیده انگلیسی

The question whether epidermal growth factor (EGF)-induced receptor endocytosis requires the prior autophosphorylation via the EGF receptor (EGFR) kinase domain has been a matter of long-standing debate. In the airway epithelial cell line NCI-H292, the EGFR kinase domain inhibitor BIBW 2948 BS was found to inhibit both autophosphorylation and subsequent internalization of the endogenous EGFR with similar IC50 values. Applying an ex vivo EGFR internalization assay in a clinical study, the in vivo effect of inhalatively administered BIBW 2948 BS was determined directly at the targeted receptor in airway tissues from COPD patients. In these experiments, the in vivo inhibition of the EGFR kinase domain prevented the EGF-induced internalization of EGFR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 317, Issue 1, 1 January 2011, Pages 42–50
نویسندگان
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