کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2131898 | 1086664 | 2007 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification and analysis of in vitro cultured CD45-positive cells capable of multi-lineage differentiation Identification and analysis of in vitro cultured CD45-positive cells capable of multi-lineage differentiation](/preview/png/2131898.png)
We report on a subset of cells that co-purify with CD45-positive/Lineage minus (CD45pos/Linminus) hematopoietic cells that are capable of in vitro differentiation into multi-potential cells including cells with neuroectoderm properties. Although these cells are CD45 positive and have properties similar to CD45-negative mesenchymal progenitor cells (MPC) derived from bone marrow (BM), they are neither hematopoietic cells nor mesenchymal cells. These CD45pos/Linminus cells can be expanded in vitro, express the stem cell genes Oct-4 and Nanog and can be induced to differentiate into endothelial cells, osteoblasts, muscle cells and neural cells at frequencies similar to those reported for bone marrow mesenchymal cells. Long-term culture of these cells followed by transplantation into NOD/SCID mice resulted in positive bone marrow stromal cell engraftment but not hematopoietic engraftment, suggesting that despite their CD45-positive status these cells do not have the same properties as hematopoietic stem cells. Clonal cell analysis determined that the culture period caused a broadening in the differentiation potential of the starting population.
Journal: Experimental Cell Research - Volume 313, Issue 9, 15 May 2007, Pages 1839–1852