کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2131907 1086664 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Sensitive detection of GFP utilizing tyramide signal amplification to overcome gene silencing
چکیده انگلیسی

The green fluorescent protein (GFP) is among the most commonly used expression markers in biology. GFP-tagged cells have played a particularly important role in studies of cell lineage. Sensitive detection of GFP is crucially important for such studies to be successful, and problems with detection may account for discrepancies in the literature regarding the possible fate choices of stem cells. Here we describe a very sensitive technique for visualization of GFP. Using it we can detect about 90% of cells of donor origin while we could only see about 50% of these cells when we employ the methods that are in general use in other laboratories. In addition, we provide evidence that some cells permanently silence GFP expression. In the case of the progeny of bone marrow stem cells, it appears that the more distantly related they are to their precursors, the more likely it is that they will turn off the lineage marker.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 313, Issue 9, 15 May 2007, Pages 1943–1950
نویسندگان
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