کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2131945 1086666 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hsp70B′ and Hsp72 form a complex in stressed human colon cells and each contributes to cytoprotection
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Hsp70B′ and Hsp72 form a complex in stressed human colon cells and each contributes to cytoprotection
چکیده انگلیسی

Hsp70B′ is the only major human isoform in the hsp70 family that is strictly stress-inducible, and therefore available to function only in stressed cells. Since Hsp70B′ is evolutionarily closely related to human Hsp72, they are thought to function similarly, but direct evidence of Hsp70B′ function in stressed cells has been lacking. Here we showed that both Hsp70B′ and Hsp72 are essential relatively early after heat stress in the acquisition of cytoprotection by two human colon cell lines. Using flow cytometry to count viable cells, we also showed that cytoprotection is more pronounced in cultures grown at low cell number (LCN), where there is an ample amount of both Hsp70s. siRNA knock-down of either Hsp70B′ or Hsp72 severely handicapped the ability of cells to acquire cytoprotection. Hsp70B′ and Hsp72 were found to form a complex following stress that included the co-chaperone HOP. These results taken together support the hypothesis that Hsp70B′ and Hsp72 play cooperative roles in cell survival of proteotoxic stress. In addition there are implications for chemotherapy protocols and for pathological conditions in which the contributions to cytoprotection of both Hsp70B′ and Hsp72 are modulated by cell numbers or density.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 314, Issue 13, 1 August 2008, Pages 2468–2476
نویسندگان
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