کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2132748 1086714 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a population of cells with hematopoietic stem cell properties in mouse aorta–gonad–mesonephros cultures
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Identification of a population of cells with hematopoietic stem cell properties in mouse aorta–gonad–mesonephros cultures
چکیده انگلیسی

The aorta–gonad–mesonephros (AGM) region is a primary source of definitive hematopoietic cells in the midgestation mouse embryo. In cultures of dispersed AGM regions, adherent cells containing endothelial cells are observed first, and then non-adherent hematopoietic cells are produced. Here we report on the characterization of hematopoietic cells that emerge in the AGM culture. Based on the expression profiles of CD45 and c-Kit, we defined three cell populations: CD45low c-Kit+ cells that had the ability to form hematopoietic cell colonies in methylcellulose media and in co-cultures with stromal cells; CD45low c-Kit− cells that showed a granulocyte morphology; CD45high c-Kitlow/− that exhibited a macrophage morphology. In co-cultures of OP9 stromal cells and freshly prepared AGM cultures, CD45low c-Kit+ cells from the AGM culture had the abilities to reproduce CD45low c-Kit+ cells and differentiate into CD45low c-Kit− and CD45high c-Kitlow/− cells, whereas CD45low c-Kit− and CD45high c-Kitlow/− did not produce CD45low c-Kit+ cells. Furthermore, CD45low c-Kit+ cells displayed a long-term repopulating activity in adult hematopoietic tissue when transplanted into the liver of irradiated newborn mice. These results indicate that CD45low c-Kit+ cells from the AGM culture have the potential to reconstitute multi-lineage hematopoietic cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 313, Issue 5, 10 March 2007, Pages 965–974
نویسندگان
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