کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2144579 | 1088387 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hedgehog signaling in small-cell lung cancer: Frequent in vivo but a rare event in vitro
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کلمات کلیدی
SufuSMORNA interference - RNA تداخل کنندهSmall interfering RNA - RNA تداخل کوچکRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsiRNA - siRNASCLC - بگذارندHedgehog - جوجهتیغی، خارپشتShh - خیرSmall-cell lung cancer - سرطان ریه کوچک سلولیNSCLC - سرطان ریوی غیر سلول کوچکsuppressor of fused - سرکوب کننده همجوشیcyclopamine - سیکلوپامینSmoothened - صافsonic hedgehog - صدای جیر جیرHedgehog signaling pathway - مسیر سیگنالینگ یخچالHhip - هیپHedgehog interacting protein - پروتئین تعامل یوگاPtch - پچPatched - پچ شده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The hedgehog (HH) signaling pathway plays multiple roles during embryonic development and increasing evidence suggests that this embryonic pathway is involved in development and progression of several human cancers including those of the brain, skin, lung and gastrointestinal tract. To investigate HH signaling activity in small-cell lung cancer (SCLC), we have performed gene expression analysis on members of the HH pathway on a panel of 20 SCLC cell lines. Sonic hedgehog (SHH) expression was detected in only DMS79 and GLC16 and only DMS114 expressed detectable protein levels of GLI1, one of the key transcription factors of the pathway. Involvement of HH signaling in SCLC proliferation was investigated in a subset of cell lines using the HH signaling inhibitor cyclopamine or small interfering RNA (siRNA) against GLI1. Cells expressing GLI1 responded only weakly to both cyclopamine and RNA interference, suggesting that HH signaling plays only a minor role in the growth of SCLC cell lines. To investigate HH pathway activity in vivo, GLI1 immunohistochemistry was performed on SCLC tumors. Interestingly, GLI1 was expressed in most SCLC tumors studied, indicating that HH signaling is important for in vivo growth of SCLC but establishment of cell lines from SCLC tumors may lead to loss of expression of key HH pathway members. Thus, the data support the idea that the HH pathway may be a therapeutic target in SCLC. However, the data also suggest that the SCLC cells can circumvent the apparent in vivo requirement of HH signaling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Lung Cancer - Volume 52, Issue 3, June 2006, Pages 281-290
Journal: Lung Cancer - Volume 52, Issue 3, June 2006, Pages 281-290
نویسندگان
Janni Vestergaard, Mikkel W. Pedersen, Nina Pedersen, Christian Ensinger, Zeynep Tümer, Niels Tommerup, Hans Skovgaard Poulsen, Lars Allan Larsen,