Aptasensor platform based on carbon nanofibers enriched screen printed electrodes for impedimetric detection of thrombin

• Electrochemical aptasensor was developed by using carbon nanofibers enriched screen printed electrodes (CNF-SPEs).
• The surface morphologies of CNF-SPE were explored using SEM analysis.
• DNA aptamer recognizing thrombin (THR) was immobilized onto the surface of CNF-SPEs.
• The selectivity to THR was tested in the presence of random DNA sequences or random aptamers.
• The impedimetric analysis of THR and selectivity of the aptasensor was also studied in artificial serum.

Herein, impedimetric detection of THR could be achieved using an aptasensing platform based on carbon nanofibers enriched screen-printed electrodes (CNF-SPE). The resistance to charge transfer (Rct) was recorded using electrochemical impedance spectroscopy (EIS) technique before/after the immobilization of amino-modified DNA aptamer (APT) selective to thrombin (THR) onto the surface of CNF-SPEs and the specific interaction between APT and THR. The selectivity of the aptasensor was also tested in the presence of a random DNA oligonucleotide and a DNA aptamer that were different from THR specific APT. The impedimetric aptasensing of target protein was also explored in the fetal bovine serum (FBS) medium at different concentration levels of THR. Additionally, the selectivity of the aptasensor was tested against bovine serum albumin (BSA) and protein C (PC) in FBS medium. This CNF-SPE based aptasensor platform allows a reliable, sensitive and selective impedimetric monitoring of THR.

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