Immobilization of glucose oxidase on reagentless ferrocene-containing polythiophene derivative and its glucose sensing application

A novel polymer electrode in the form of a thin film was prepared by electrochemical copolymerization of thiophene, thiophene-3-acetic acid, and dicyclopentadienyl iron-1,4-dienylmethyl-2-(thiophen-3-yl)acetate, for fabricating GOx-immobilized electrodes for amperometric sensing of glucose. Glucose oxidase was immobilized by covalent binding to the carboxyl groups on the electrode. The catalytic electrochemistry of the enzyme electrode with the copolymer was investigated. The enzyme electrodes act as a mediator between the redox center of glucose oxidase (GOx) and the electrode.