Effect of biosynthesized silver nanoparticles on the growth and some biochemical parameters of @@ Aspergillus foetidus

• AgNPs affects the growth of @@ Aspergillus foetidus.
• Microscopic study showing adherence of AgNPs on @@ A. foetidus.
• Alteration of catalase activity, lipid peroxidation, thiol, and protein content in presence of AgNPs.

The biosynthesis of silver nanoparticles (AgNPs) has been carried out by using the extracellular filtrate of the fungal strain, @@ Aspergillus foetidus. The synthesized nano particles have been characterized following different biophysical techniques. The results obtained from the studies of antifungal activities of AgNPs were found to be of utmost significance. Growth of @@ A. foetidus both in liquid and solid CD media have been monitored in presence of the nanoparticles (0–40 ppm). Medium free dry biomass of the fungi was collected and emphasis has been laid on the assay of catalase activity, lipid per oxidation, thiol and protein content estimation and leakage of thiol, protein content. Growth of fungi in the presence of AgNPs was significantly inhibited in a dose dependent manner. Adherence of AgNPs on the fungal strain was realized by field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy, which indicated presence of silver nanomaterials in the surface of fungal mycelium. The growth of the fungus was found to be remarkably decreased with the increase in concentration of AgNPs and a considerable change in the activities and contents of all of the biochemical parameters considered indicated a plausible mechanistic mode of action of biosynthesized AgNPs establishing its antifungal activity.

Growth of @@ A. foetidus both in liquid and solid Czapek Dox media have been studied in presence of AgNPs. The growth of the fungus was found to be remarkably decreased with the increase of concentration of AgNPs and it was found that the activities of enzymes and contents of all of the biochemical parameters (assay of catalase, lipid per oxidation, thiol & protein content) were found to be changed which may be considered as a possible mechanistic of mode of action of antifungal activity of biosynthesized AgNPs.Figure optionsDownload as PowerPoint slide