Extraction of peroxidase from waste Brassica oleracea used for the treatment of aqueous phenol in synthetic waste water

• Peroxidase was extracted and purified from waste cauliflower stem and leaf extract.
• Enzyme activity was found to be greater for stem than leaves.
• Phenol removal was achieved using cauliflower stem peroxidase.
• PEG addition reduced the inactivation of cauliflower peroxidase.

In this study, peroxidase was extracted from waste cauliflower stem and leaf and was partially purified. Peroxidase activity of cauliflower stem extract was found to be greater than that in leaf extract. Optimal temperature, pH and ionic strengths for cauliflower stem extract were found to be using pyrogallol/H2O2 substrate and used to catalyze the oxidation and polymerization of phenolic compounds in synthetic waste water. Further, the effect of H2O2, enzyme concentration, initial phenol and polyethylene glycol (PEG) concentrations was investigated on the phenol removal process. In the presence of PEG the phenol removal increased dramatically from 35% to >90% and FT-IR study on phenol samples before and after the peroxidase treatment showed the phenol radical formation and a decrease in phenol concentration.

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