کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
22290 43267 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid conversion of toluene by an acinetobacter sp. Tol 5 mutant showing monolayer adsorption to water-oil interface
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Rapid conversion of toluene by an acinetobacter sp. Tol 5 mutant showing monolayer adsorption to water-oil interface
چکیده انگلیسی

Two-liquid-phase partitioning bioreactors (TPPBs) consist of an aqueous phase containing microorganism cells and a water-immiscible, non-bioavailable organic phase, and have been investigated for use in bioconversion of toxic, hydrophobic chemicals. We achieved a toluene conversion rate 10-fold faster than any experimental data previously demonstrated, using a novel bacterium in a TPPB employing silicone oil. The bacterium was a bald mutant of toluene-degrading Acinetobacter sp. Tol 5, which has numerous filamentous cell appendages, hydrophobic cell surfaces, adheres to solid surfaces, and self-agglutinates. The bald mutant cells lack the appendages, have decreased adhesiveness to solid surfaces, retain the hydrophobic cell surface, and reversibly adsorb to the surface of oil droplets dispersed in an aqueous phase. The effects of toluene concentration, aqueous and oil phase volume ratios, and cell concentration on the initial conversion rate of toluene were investigated. Volume ratios did not significantly affect conversion rate, and the rate greater than 1 g/(l·h) was attained even in W/O emulsion containing 80% oil. As cell concentration increased, the volumetric conversion rate increased, but the specific conversion rate decreased. The highest volumetric conversion rate, 3.1 g/(l·h), was achieved using 8.20 g/l cells in a TPPB containing 20% oil.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 3, September 2008, Pages 226–230
نویسندگان
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