Studies on inactivation of Bacillus subtilis spores by high hydrostatic pressure and heat using design of experiments

The results of fractional factorial experiments showed that the significant external factors affecting high-pressure processing (HPP) inactivation were pressure, temperature and pressure holding time. Based on these results, response surface methodology (RSM) was employed in the present work and a quadratic equation for HPP inactivation was built. By analyzing the response surface plots and their corresponding contour plots as well as solving the quadratic equation, the experimental values were shown to be significantly in good agreement with predicted values since the adjusted determination coefficient (RAdj2) was 0.9384. The optimum process parameters for six log-cycles reduction of Bacillus subtilis spores were obtained as: temperature, 87 °C; pressure, 576.0 MPa and pressure holding time, 13 min. The adequacy of the model equation for predicting the optimum response values was verified effectively by the validation data.