کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
23061 | 43415 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture](/preview/png/23061.png)
چکیده انگلیسی
• UV-A and blue light-inducible gene expression system.
• Mammalian synthetic light-dependent dimerization of CRY2 and C1BN.
• Cell-based evaluation of sun cream UV-A protection properties.
Light-dependent gene regulation systems are advantageous as they allow for precise spatio-temporal control of target gene expression. In this paper, we present a novel UV-A and blue-light-inducible gene control system that is based on the light-dependent heterodimerization of the CRY2 and C1BN domains. Upon their interaction, a transcription factor is released from the cell membrane and initiates target gene expression. Capitalizing on that, sun cream UV-A protection properties were measured intracellularly.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 189, 10 November 2014, Pages 150–153
Journal: Journal of Biotechnology - Volume 189, 10 November 2014, Pages 150–153
نویسندگان
Markus Wieland, Marius Müller, Andreas Kyburz, Phillip Heissig, Sebastian Wekenmann, Franziska Stolz, Simon Ausländer, Martin Fussenegger,