کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
23295 43430 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tuning cell cycle of insect cells for enhanced protein production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Tuning cell cycle of insect cells for enhanced protein production
چکیده انگلیسی


• We identified the complete cyclin E (cycE) cDNA sequence from industrially relevant Trichoplusia ni (T. ni) derived High Five™ genomes, cloned the gene, and expressed and purified the protein.
• We overexpressed cyclin E and found enhanced growth rate owing to additional cyclin E.
• We employed RNAi to suppress cycE gene expression and found diminished cell growth.
• We employed RNAi to “tune” cell cycle by arresting cells in G1 and doubled the yield of recombinant protein, as indicated by GFP expression.
• The effect of RNAi on protein yield was found to be dose-dependent.

The eukaryotic cell cycle consists of many checkpoints during which certain conditions must be met before passing to subsequent stages. These safeguards ensure cells’ integrity and survival, but may also limit growth and protein synthesis in protein production processes. In this work, we employ metabolic engineering principles to “tune” the cell cycle to overcome checkpoint processes in order to facilitate faster cell growth, and independently, arrest the cell cycle in gap1 (G1) phase for greater protein productivity. Specifically, we identified the complete cyclin E (cycE) cDNA sequence from industrially relevant, Trichoplusia ni (T. ni) derived High Five™ genomes. We then both knocked down (through RNA interference; RNAi) and overexpressed (on an expression plasmid) cycE gene expression to tune the cell phenotype. We successfully up- and down-regulated cycE transcription, enhancing and hindering cell growth, respectively. We also measured the effects of titrated cycE expression on the cell cycle phase distribution. Finally, we investigated the dose-dependent effects of dsCycE on recombinant protein production using the baculovirus expression system and demonstrated a nearly 2-fold increase in expression of model protein (GFPuv).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 168, Issue 1, 10 October 2013, Pages 55–61
نویسندگان
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