کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
23692 43463 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An explicit expression for determining cometabolism kinetics using progress curve analysis
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
An explicit expression for determining cometabolism kinetics using progress curve analysis
چکیده انگلیسی

We present an explicit expression for describing the kinetics of cometabolic biotransformation of environmental pollutants. This expression is based on the Lambert W function and explicitly relates the substrate concentration, S, to time, t, the two experimentally measured variables. This explicit relationship simplifies kinetic parameter estimation as differential equation solution and iterative estimation of the substrate concentration are eliminated. The applicability of this new expression for nonlinear kinetic parameter estimation was first demonstrated using noise containing synthetic data where final estimates of the kinetic parameters were very close to their actual values. Subsequently 1.1.1-trichloroethane degradation data at initial concentrations of 750 and 375 μM were described using the explicit expression resulting in r and Ks estimates of 0.26 μM/mg d and 28.08 μM and 0.30 μM/mg d and 28.70 μM, respectively, very similar to 0.276 μM/mg d and 31.2 μM, respectively, that were reported in the original study. The new explicit expression presented in this study simplifies estimation of cometabolic kinetic parameters and can be easily used across all computational platforms thereby providing an attractive alternative for progress curve analysis.


► An explicit expression for describing cometabolism kinetics is presented.
► This eliminates numerical and iterative estimation of the substrate concentration.
► The applicability was demonstrated using both synthetic and experimental data.
► This simplified approach can be implemented across most computational platforms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 159, Issues 1–2, 31 May 2012, Pages 56–60
نویسندگان
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