کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
23708 | 43465 | 2012 | 8 صفحه PDF | دانلود رایگان |

White spot syndrome virus is currently the leading cause of production losses in the shrimp industry. Penaeus monodon Rab7 protein has been recognized as a viral-binding protein with an efficient protective effect against white spot syndrome infection. Plant-derived recombinant PmRab7 might serve as an alternative source for in-feed vaccination, considering the remarkable abilities of plant expression systems. PmRab7 was introduced into the Arabidopsis thaliana T87 genome. Arabidopsis-derived recombinant PmRab7 showed high binding activity against white spot syndrome virus and a viral envelope, VP28. The growth profile of Arabidopsis suspension culture expressing PmRab7 (ECR21# 35) resembled that of its counterpart. PmRab7 expression in ECR21# 35 reached its maximum level at 5 mg g−1 dry weight in 12 days, which was higher than those previously reported in Escherichia coli and in Pichia. Co-injection of white spot syndrome virus and Arabidopsis crude extract containing PmRab7 in Litopenaeus vannamei showed an 87% increase in shrimp survival rate at 5 day after injection. In this study, we propose an alternative PmRab7 source with higher production yield, and cheaper culture media costs, that might serve the industry's need for an in-feed supplement against white spot syndrome infection.
► Arabidopsis-derived rPmRab7 is an alternative source of WSSV protective protein.
► AtrPmRab7 production (5 mg g−1 DW) was higher than previously reported in Pichia.
► Co-injection of AtrPmRab7 and WSSV increased Peneaus vannamei survival rate by 87%.
► AtrPmRab7 showed feasibility to serve industrial needs for shrimp feed supplement.
Journal: Journal of Biotechnology - Volume 161, Issue 1, 15 September 2012, Pages 60–67