کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2402190 1102667 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Classical swine fever virus marker vaccine strain CP7_E2alf: Shedding and dissemination studies in boars
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Classical swine fever virus marker vaccine strain CP7_E2alf: Shedding and dissemination studies in boars
چکیده انگلیسی


• Shedding and distribution in boar was assessed for CSFV vaccine strain CP7_E2alf.
• Primary replication in lymphatic organs was confirmed.
• Complete lack of CP7_E2alf detection was shown in genitals and accessory sex glands.
• No shedding in urine, faeces, or saliva occurred.
• The study further substantiates safety of marker vaccine strain CP7_E2alf.

Over the last decade, pestivirus chimaera CP7_E2alf has proven to be a most promising marker vaccine candidate against classical swine fever (CSF). To provide further background data for the risk assessment towards licensing and release, especially on presence of the vaccine chimaera in faeces, urine, and organs of the male reproductive tract, supplementary studies were carried out under controlled laboratory conditions. In detail, the shedding and dissemination pattern of Suvaxyn® CSF Marker (“CP7_E2alf”) was assessed in 12 adult boars after single intramuscular vaccination with a tenfold vaccine dose. Four and seven days post vaccination, six animals were subjected to necropsy and triplicate samples were obtained from reproductive and lymphatic organs as well as urine, faeces, blood, and several additional organs and matrices. The sampling days were chosen based on pre-existing data that indicated the highest probability of virus detection. Upon vaccination, neither local nor systemic adverse effects were observed in the experimental animals. It was confirmed that primary replication is restricted to the lymphatic tissues and especially the tonsil. While viral genome was detectable in several samples from lymphatic tissues at four and seven days post vaccination, infectious virus was only demonstrated at four days post vaccination in one tonsil sample and one parotid lymphnode. Sporadic detection at a very low level occurred in some replicates of liver, lung, bone marrow, and salivary gland samples. In contrast, viral genome was not detected in any sample from reproductive organs and accessory sex glands, in faeces, urine, or bile.The presented data on the dissemination of the vaccine virus CP7_E2alf in adult boars are supplementing existing safety and efficacy studies and indicate that the use of the vaccine is also safe in reproductive boars.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 33, Issue 27, 17 June 2015, Pages 3100–3103
نویسندگان
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