کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2403184 | 1102887 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunogenicity assay validation for an HIV vaccine trial: High IFNγ+/IL-2+ CD8+ T cells background in healthy Thais
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
In a vaccine trial, assays for vaccine immunogenicity if performed locally will strengthen local site, can save costs and avoid hurdles associated with specimen transport. Here we report the optimization and validation of an Intracellular Cytokine Staining (ICS) assay which was undertaken in preparation for a phase I HIV vaccine trial conducted in Thailand. Intra-, and inter-operator variability were easily established. However, while attempting to set population cut offs for a positive response we found 4/36 (11%) high background responses of IFNγ+ and/or IL-2+ CD8+ T cells (>1%) in normal healthy volunteers. The determinates of these unexpected responses were explored and minimized.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 29, Issue 35, 11 August 2011, Pages 6002–6007
Journal: Vaccine - Volume 29, Issue 35, 11 August 2011, Pages 6002–6007
نویسندگان
Sunee Sirivichayakul, Pattarawat Thantiworasit, Pornsupa Chatkulkawin, Supranee Buranapraditkun, Mee Ling Munier, Anthony D. Kelleher, Kiat Ruxrungtham,