کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2404069 | 1102951 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Modified binding assay for improved sensitivity and specificity in the detection of residual pertussis toxin in vaccine preparations
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Modified binding assay for improved sensitivity and specificity in the detection of residual pertussis toxin in vaccine preparations Modified binding assay for improved sensitivity and specificity in the detection of residual pertussis toxin in vaccine preparations](/preview/png/2404069.png)
چکیده انگلیسی
A binding assay was recently published that differentiates between pertussis toxin (PTx) and the pertussis toxoid (PTd) used in acellular pertussis vaccines based on the selective binding of PTx to fetuin and detection with a polyclonal antibody. We found that the assay specificity for PTx was affected by both pH and salt. A monoclonal antibody (mAb) was identified that eliminated specificity problems and improved the sensitivity to 0.4 ng PTx/ml. This mAb was previously shown to neutralize PTx toxicity in vivo, thereby supporting the assay's potential biological relevance as an alternative to the mouse histamine sensitivity test for the safety of pertussis vaccines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 28, Issue 15, 24 March 2010, Pages 2687–2692
Journal: Vaccine - Volume 28, Issue 15, 24 March 2010, Pages 2687–2692
نویسندگان
Richard A. Isbrucker, Alex Bliu, Fiona Prior,