کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2404902 1103004 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immune response of horses to vaccination with the recombinant Hc domain of botulinum neurotoxin types C and D
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Immune response of horses to vaccination with the recombinant Hc domain of botulinum neurotoxin types C and D
چکیده انگلیسی

Botulinum neurotoxins, predominantly serotypes C and D, cause equine botulism through forage poisoning. The C-terminal part of the heavy chain of botulinum neurotoxin types C and D (HcBoNT/C and D) was expressed in Escherichia coli and evaluated as a recombinant mono- and bivalent vaccine in twelve horses in comparison to a commercially available toxoid vaccine. A three-dose subcutaneous immunization of adult horses elicited robust serum antibody response in an ELISA using the immunogen as a capture antigen. Immune sera showed dose-dependent high potency in neutralizing specifically the active BoNT/C and D in the mouse protection assay. The aluminium hydroxide based mono- and bivalent recombinant HcBoNT/C and D vaccines were characterized by good compatibility and the ability to elicit protective antibody titers similar or superior to the commercially available toxoid vaccine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 27, Issue 41, 18 September 2009, Pages 5661–5666
نویسندگان
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