کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2405310 | 1103030 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of recombinant influenza A virus as a vector for HIV-1 p17Gag Characterization of recombinant influenza A virus as a vector for HIV-1 p17Gag](/preview/png/2405310.png)
We have generated a recombinant influenza A virus with the HIV-1 p17Gag (rFlu-p17) gene inserted into the influenza virus neuraminidase (NA) gene. Expression of HIV-1 p17 protein was detected by conventional Western blot analysis and also by liquid chromatography/tandem mass spectrometry (LC–MS/MS) analysis of rFlu-p17 infected cells. The latter method does not depend on protein-specific antibody preparations and proved to be a powerful tool to detect proteins of interest. Next, antigen presentation of p17 expressed after infection of antigen-presenting cells was determined. Cloned p17-specific CD8+ T-cells were co-cultured with rFlu-p17 infected B-cells and produced IFN-γ upon stimulation. Furthermore, we showed that immunization with rFlu-p17 elicited a humoral immune response in mice. This study shows that replication-deficient rFlu-p17 is antigenic in vitro and immunogenic in vivo and warrants further development as a candidate vaccine vector.
Journal: Vaccine - Volume 27, Issue 42, 25 September 2009, Pages 5735–5739