کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2405907 1103054 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Suspension-Vero cell cultures as a platform for viral vaccine production
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Suspension-Vero cell cultures as a platform for viral vaccine production
چکیده انگلیسی

Since Vero cells are currently considered as an acceptable cell substrate to produce a wide range of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in serum-free media. After adapting anchorage-dependent Vero cells to grow as a free-cell suspension, vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 production rates were evaluated in batch cultures using spinner flasks and perfused cultures in a bioreactor. The achieved results constitute valuable information for the development of a low-cost high-productivity process using a suspension culture of Vero cells to produce viral vaccines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 27, Issue 46, 30 October 2009, Pages 6464–6467
نویسندگان
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