کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2405946 1103056 2008 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Physicochemical characterisation of glycoconjugate vaccines for prevention of meningococcal diseases
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Physicochemical characterisation of glycoconjugate vaccines for prevention of meningococcal diseases
چکیده انگلیسی

SummaryBacterial capsular polysaccharides covalently linked to an appropriate carrier protein represent the best tool to induce a protective immune response against a wide range of bacterial diseases, such as meningococcal infections. We describe here the physico-chemical characterisation of glycoconjugate molecules designed to prepare a vaccine against Neisseria meningitidis serogroups A, C, W135 and Y. The use of a selective conjugation chemistry resulted in well characterised, reproducible and traceable glycoconjugate that can be consistently manufactured at large scale.A pool of physical and spectroscopic methods was used to establish glycosylation ratio, identity, molecular weight profiles, integrity of carrier protein and sites of glycosylation, assuring effective and consistent lots of vaccines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 26, Issue 18, 24 April 2008, Pages 2284–2296
نویسندگان
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