کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2406791 1103096 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activation of dendritic cells and induction of CD4+ T cell differentiation by aluminum-containing adjuvants
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Activation of dendritic cells and induction of CD4+ T cell differentiation by aluminum-containing adjuvants
چکیده انگلیسی

Aluminum-containing adjuvants are widely used in licensed human and veterinary vaccines. However, the mechanism by which these adjuvants enhance the immune response and predominantly stimulate a TH2 humoral immune response is not well understood. In this study, the effects of aluminum hydroxide and aluminum phosphate adjuvants on antigen presentation, expression of costimulatory molecules and cytokines by mouse dendritic cells (DCs) and the ability of DCs to induce T helper cell differentiation were investigated. Dendritic cells pulsed with ovalbumin (OVA) adsorbed to aluminum-containing adjuvants activated antigen-specific T cells more effectively than DCs pulsed with OVA alone. Aluminum hydroxide adjuvant had a significantly stronger effect than aluminum phosphate adjuvant. Both aluminum-containing adjuvants significantly increased the expression of CD86 on DCs but only aluminum hydroxide adjuvant also induced moderate expression of CD80. Aluminum-containing adjuvants stimulated the release of IL-1β and IL-18 from DCs via caspase-1 activation. DCs incubated with LPS and OVA induced TH1 differentiation of naïve CD4+ T cells. In contrast, DCs incubated with aluminum/OVA activated CD4+ T cells to secrete IL-4 and IL-5 as well as IFN-γ. Addition of neutralizing anti-IL-1β antibodies decreased IL-5 production and addition of anti-IL-18 antibodies decreased both IL-4 and IL-5 production. Inhibition of IL-1β and IL-18 secretion by DCs via inhibition of caspase-1 also led to a marked decrease of IL-4 and IL-5 by CD4+ T cells. These results indicate that aluminum-containing adjuvants activate DCs and influence their ability to direct TH1 and TH2 responses through the secretion of IL-1β and IL-18.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 23, 6 June 2007, Pages 4575–4585
نویسندگان
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