کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2407816 1103143 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Development and preclinical evaluation of an alphavirus replicon particle vaccine for cytomegalovirus
چکیده انگلیسی

We used a replication-incompetent, single-cycle, alphavirus replicon vector system to produce virus-like replicon particles (VRP) expressing the extracellular domain of human cytomegalovirus (CMV) glycoprotein B or a pp65/IE1 fusion protein. Efficient production methods were scaled to produce pilot lots and clinical lots of each alphavirus replicon vaccine component. The vaccine induced high-titered antibody responses in mice and rabbits, as measured by ELISA and CMV neutralization assays, and robust T-cell responses in mice, as measured by IFN-γ ELISPOT assay. A toxicity study in rabbits showed no adverse effects in any toxicology parameter. These studies support clinical testing of this novel CMV alphavirus replicon vaccine in humans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 42, 16 October 2007, Pages 7441–7449
نویسندگان
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