Production of monoclonal antibodies against whole virus particles of foot-and-mouth disease virus serotype O and A and their potential use in quantification of intact virus for vaccine manufacture

A double antibody sandwich ELISA for quantification of foot-and-mouth disease (FMD) whole virus particle was developed using two monoclonal antibodies (mAbs) against FMD virus (FMDV) serotype O and A. The antibody binding epitopes were characterized. The two mAbs reacted with all of the tested FMDV strains. The use of these two mAbs in the sandwich ELISA will allow the quantification of FMD whole virus particles during vaccine manufacture.