کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2408669 1551787 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA
چکیده انگلیسی

An ELISA assay was developed to allow the rapid and accurate identification of human influenza A N1 and N2 neuraminidases. Initial testing using a fetuin pre-coating of wells correctly identified 81.7% of the neuraminidase type from a series of human A(H1N1), A(H1N2) and A(H3N2) viruses. This result could be improved to detect the neuraminidase subtype of almost all human influenza A viruses from a large panel of viruses isolated from 2000 to 2005, if the fetuin pre-coating was removed and the viruses were coated directly onto wells. This method is simple, rapid and can be used to screen large numbers of currently circulating human influenza A viruses for their neurraminidase subtype and is a good alternative to RT-PCR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 24, Issues 44–46, 10 November 2006, Pages 6675–6678
نویسندگان
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