کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2409945 1103239 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
GM-CSF-surface-modified B16.F10 melanoma cell vaccine
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
GM-CSF-surface-modified B16.F10 melanoma cell vaccine
چکیده انگلیسی

Through the specific and tight interaction between streptavidin and biotin, a novel platform was developed to allow for rapid (less than 2 h), efficient and durable display of streptavidin-tagged bioactive GM-CSF on the surface of biotinylated B16.F10 tumor cells. This technology involved biotinylation of the cell membrane with a biotin derivative and surface modification of the biotinylated cells with the bi-functional fusion protein, streptavidin-tagged GM-CSF. Furthermore, the resultant GM-CSF-modified B16.F10 whole tumor cell vaccine could induce strong and long-lasting systemic protection against the wild-type tumor challenge. Therefore, the platform may represent a fast, efficient and safe approach for the whole tumor cell vaccination.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 24, Issue 25, 19 June 2006, Pages 5265–5268
نویسندگان
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