کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2410263 1103254 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Haemagglutinin quantification and identification of influenza A&B strains propagated in PER.C6® cells: A novel RP-HPLC method
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Haemagglutinin quantification and identification of influenza A&B strains propagated in PER.C6® cells: A novel RP-HPLC method
چکیده انگلیسی

The major antigenic determinant of influenza A and B virus is haemagglutinin (HA). The HA content is an important specification of influenza vaccines. HA in vaccines has typically been quantified by single-radial-immunodiffusion (SRID). However, SRID is a laborious and low throughput assay. Moreover, sensitivity, accuracy, and precision, especially for non-purified (in-process) influenza virus is relatively low. We present a novel method for quantification of HA in influenza viral cultures as well as for the identification of HA from individual influenza strains in trivalent vaccines.The method is based on the separation of HA1, the hydrophilic subunit of HA, from the more hydrophobic viral and matrix components by reversed-phase high performance liquid chromatography (RP-HPLC). The HA1 peak area is demonstrated to be proportional to the level of HA in non-purified, semi-purified and purified vaccine products of various epidemic and pandemic influenza A and B strains propagated in PER.C6® cell cultures. The RP-HPLC assay selectivity allows for the simultaneous identification and quantification of HA1 from influenza A and B strains in the yearly revised trivalent vaccines for epidemic outbreaks.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 24, Issue 16, 12 April 2006, Pages 3137–3144
نویسندگان
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