کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2410422 | 1103259 | 2006 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficiency of HPV 16 L1/E7 DNA immunization: Influence of cellular localization and capsid assembly
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Infections by human papillomaviruses (HPV) are the major cause of uterine cancer in women worldwide. Aiming to develop a combined prophylactic and therapeutic vaccine we have previously demonstrated immunogenicity of chimeric virus-like particles consisting of a C-terminally truncated HPV 16 L1 capsid protein fused to an E7 portion. Here we show that genetic vaccination with a corresponding DNA was inefficient in the induction of a L1-specific prophylactic immune response. DNA immunization with C-terminally truncated HPV 16 L1 genes of different lengths revealed that only short deletions (L11-498) were tolerated for eliciting a humoral immune response against viral capsids. This correlates with the observation that the C-terminal sequences are critical for nuclear localization, capsomere and capsid assembly. However, only the ability of L1 protein to form capsomeres or capsids showed a direct influence on the outcome of the immune response. C-terminal insertion of 60 amino acids of E7 was tolerated in fusion constructs, whereas insertion of full-length E71-98 or shuffled E7 (149 aa) completely abolished the humoral immune response. The L11-498/E71-60 fusion construct not only induced L1-specific antibodies but also L1- and E7-specific CTL responses after DNA vaccination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 24, Issue 15, 5 April 2006, Pages 2952-2965
Journal: Vaccine - Volume 24, Issue 15, 5 April 2006, Pages 2952-2965
نویسندگان
Dirk Kuck, Christoph Leder, Andrea Kern, Martin Müller, Konrad Piuko, Lutz Gissmann, Jürgen A. Kleinschmidt,