کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2410739 1103275 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modified anthrax fusion proteins deliver HIV antigens through MHC Class I and II pathways
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Modified anthrax fusion proteins deliver HIV antigens through MHC Class I and II pathways
چکیده انگلیسی

T cell-based HIV vaccine candidates have focused on eliciting both CD4- and CD8-mediated responses. One challenge in vaccine development is the successful introduction and presentation of exogenous antigen to elicit an immune response. Modified bacterial toxins have been studied extensively as intracellular delivery agents because of their unique capability to translocate antigen across the cell membrane without affecting cell viability. Modified anthrax toxin lethal factor (LFn) fusion protein is able to effectively induce anti-HIV cytotoxic T lymphocytes in the absence of protective antigen (PA) and is being evaluated as a vaccine candidate. Here we describe, for the first time, the processing and presentation of LFn fusion proteins by the MHC Class II pathway. The ability of LFn–HIV to induce both CD8- and CD4-mediated responses may have relevance in current approaches to vaccine design. Furthermore, the translocation and presentation of antigens occurs in the absence of PA, which proposes a modified molecular mechanism of antigen presentation by the anthrax toxin model. Additionally, we found that LFn–HIV is specific and sensitive in detecting HIV-specific CD4+ and CD8+ T cell responses in T cell assays, further broadening the value of this antigen delivery system as a useful immunologic tool.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 23, Issue 32, 14 July 2005, Pages 4128–4135
نویسندگان
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