کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2422563 | 1552888 | 2012 | 7 صفحه PDF | دانلود رایگان |
Cytokines are important regulators of the immune system and investigation of their functions may prove useful for the development of vaccines and immunostimulants for aquaculture. We therefore investigated the cytokine [interleukin (IL)-1β, IL-10, IL-12 p35 and p40, tumor necrosis factor (TNF)-α, CXC‐chemokine and interferon (IFN)-α and γ] responses of the common carp, Cyprinus carpio L., upon treatment with a commercial baker's yeast extract (CW-I) that contained nucleotides and β-glucan. Additionally, to confirm that the CW-I-induced cytokines were functional, we also assessed the effect of CW-I administration on superoxide anion production and phagocytic activities of head kidney leucocytes and resistance to Aeromonas hydrophila infection in the common carp. Our results demonstrate that baker's yeast extract-treated fish displayed a significant up-regulation of pro-inflammatory cytokine (IL-1β, IL-12 p35 and p40, TNF-α, CXC-chemokine, IFN-γ2) gene expression and a down-regulation of anti-inflammatory cytokine (IL-10) gene expression. Furthermore, significantly increased phagocytic activity and superoxide anion production in kidney cells, and resistance to a bacterial pathogen, were observed in the yeast extract-treated fish compared to non-treated fish. The current study indicates the immunostimulatory effects of a baker's yeast extract rich in nucleotides and β-glucan on the carp immune system and supports its potential use in aquaculture.
► Baker's yeast extract contained nucleotides and β-glucan as major components.
► Yeast extract-treated fish had up-regulation of cytokine genes expression.
► Treated fish showed increased phagocytosis, NBT reduction and pathogen resistance.
► Baker's yeast extract had immunostimulatory effects on common carp immune system.
► It may be used as a potential immunostimulant in carp aquaculture.
Journal: Aquaculture - Volumes 356–357, 1 August 2012, Pages 169–175