کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2423431 1552916 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro digestion of protein sources by crude enzyme extracts of the spiny lobster Panulirus argus (Latreille, 1804) hepatopancreas with different trypsin isoenzyme patterns
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
In vitro digestion of protein sources by crude enzyme extracts of the spiny lobster Panulirus argus (Latreille, 1804) hepatopancreas with different trypsin isoenzyme patterns
چکیده انگلیسی

The development of cost-effective and nutritionally adequate formulated diets is a key step in the sustainable expansion of spiny lobster aquaculture. Despite proteins are the major and most expensive component of diets, few studies are available on protein digestibility in spiny lobsters and such assessments have never been performed by in vitro methods. Two techniques were used for studying in vitro protein digestion of some common aquafeed ingredients by the spiny lobster Panulirus argus: i) the digestion cell in which the digestion products are removed by dialysis, and ii) electrophoresis which allows the visualization of the different protein fractions in the tested meals. Since three main trypsin isozyme patterns or phenotypes have been recently described in P. argus, the potential differences in protein digestion between individuals with different trypsin isozyme patterns were assessed. Results herein presented demonstrate for the first time in a crustacean species that the different trypsin phenotypes differ in protein digestion efficiency. Also, the digestion cell method was applied for the first time to a crustacea, proving to be sensitive to small changes in digestion efficiency. This method could be used in further in vitro studies for examining other aspects of spiny lobsters digestive process.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 310, Issues 1–2, 22 December 2010, Pages 178–185
نویسندگان
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