کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2425956 1552985 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cryopreservation of semen from the endangered Caspian brown trout (Salmo trutta caspius)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Cryopreservation of semen from the endangered Caspian brown trout (Salmo trutta caspius)
چکیده انگلیسی

Semen cryopreservation of the endangered Caspian brown trout (Salmo trutta caspius) and effects of two extenders and three thawing rates on fertilizing ability were studied. After assessment of semen quality, one part of semen was diluted with three parts of extender and filled into 0.5-ml straws. Freezing was carried out at 2 cm above surface of liquid nitrogen and thawed at 5 °C for 90 s, 15 °C for 45 s and 25 °C for 30 s in water baths and used for fertilization. Use of the extender: 0.3 M glucose + 10% methanol + 10% egg yolk, and 0.6 M sucrose + 10% DMSO + 10% egg yolk, yielded 66.6% ± 2.2 and 59.8% ± 5.1 eyeing rates, respectively. Thawing of cryopreserved semen was best at 25 °C water bath for 30 s and significant differences were seen in the eyeing and hatching rates between 25 °C water bath with 5 °C, 15 °C (p < 0.05). Significant interactions (p < 0.05) were found between extender type and thawing rate in this study.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 256, Issues 1–4, 15 June 2006, Pages 564–569
نویسندگان
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