کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2436129 | 1107277 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A method for rapid regulation of protein expression in Trypanosoma cruzi A method for rapid regulation of protein expression in Trypanosoma cruzi](/preview/png/2436129.png)
Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.
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► Regulation of protein expression in Trypanosoma cruzi.
► Modified pTREX vector with a ligand-controlled destabilisation domain.
► Rapid, inducible and reversible.
► Suitable for intracellular form study.
Journal: International Journal for Parasitology - Volume 42, Issue 1, January 2012, Pages 33–37