کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2436476 1107315 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Additive effects of plant expressed double-stranded RNAs on root-knot nematode development
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Additive effects of plant expressed double-stranded RNAs on root-knot nematode development
چکیده انگلیسی

Ectopically expressed double-stranded RNAs (dsRNAs) have recently been shown to suppress parasitic success of Meloidogyne spp. in plants. We have targeted two genes from the root-knot nematode Meloidogyne incognita; a dual oxidase gene implicated in the tyrosine cross-linking of the developing cuticle and a subunit of signal peptidase, a protein complex required for the processing of secreted proteins. While these genes are involved in different aspects of nematode development, the phenotypic consequences of RNA interference (RNAi) were similar with ⩾50% reduction in nematode numbers in the roots and retardation of development to the egg-producing saccate females. Expression of processed dsRNA was observed, but no evidence of detectable levels of small interfering RNAs (siRNAs) was found in the transgenic plants. We show, to our knowledge for the first time, that combining expression of these dsRNAs by crossing appropriate Arabidopsis thaliana lines resulted in an additive effect that further reduced nematode numbers and developmental capacity. Combining RNAi target genes has the potential to enhance the efficacy of RNAi and may allow control of different nematode species or genera in the crop of interest.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 40, Issue 7, June 2010, Pages 855–864
نویسندگان
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