کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2436829 | 1107359 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Preferential interaction of a 25Â kDa protein with an A6 pre-mRNA substrate for RNA editing in Trypanosoma brucei
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Preferential interaction of a 25Â kDa protein with an A6 pre-mRNA substrate for RNA editing in Trypanosoma brucei Preferential interaction of a 25Â kDa protein with an A6 pre-mRNA substrate for RNA editing in Trypanosoma brucei](/preview/png/2436829.png)
چکیده انگلیسی
Mitochondrial gene expression in kinetoplastids is controlled after transcription, potentially at the levels of RNA maturation, stability and translation. Among these processes, RNA editing by U-insertion/deletion catalysed by multi-subunit editing complexes is best characterised at the molecular level. Nevertheless, mitochondrial RNA metabolism overall remains poorly understood, including the potential regulatory factors that may interact with the relevant catalytic molecular machines and/or RNA substrates. Here we report on a â¼25Â kDa polypeptide in mitochondrial extracts that exhibits a preferential “zero-distance” photo-crosslinking interaction with an A6 pre-mRNA model substrate for RNA editing containing a single [32P] at the first editing site. The â¼25Â kDa polypeptide purified away from editosomes upon ion-exchange chromatography and glycerol gradient sedimentation. Competition assays with homologous and heterologous transcripts suggest that the preferential recognition of the A6 substrate is based on relatively low-specificity RNA-protein contacts. Our mapping and substrate truncation analyses suggest that the crosslinking activity primarily targeted a predicted stem-loop region containing the first editing sites. Consistent with the notion that pre-mRNA folding may be required, pre-annealing with guide RNA abolished crosslinking. Interestingly, this preferential protein interaction with the A6 substrate seemed to require adenosine 5â²-triphosphate but not hydrolysis. As in other biological systems, fine regulation in vivo may be brought about by transient networks of relatively low-specificity interactions in which multiple auxiliary factors bind to mRNAs and/or editing complexes in unique higher-order assemblies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 36, Issue 12, October 2006, Pages 1295-1304
Journal: International Journal for Parasitology - Volume 36, Issue 12, October 2006, Pages 1295-1304
نویسندگان
Kari Halbig, Anastasia Sacharidou, Monica De Nova-Ocampo, Jorge Cruz-Reyes,