کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
24377 43510 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone—Development, scope and limitations
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Fluorogenic substrates for the screening assay of transketolase through beta-elimination of umbelliferone—Development, scope and limitations
چکیده انگلیسی

5-O-Coumarinyl-d-xylulose was studied as a fluorogenic substrate for the stereospecific assay of transketolase enzyme. Enzymatic C2–C3 cleavage released an α-hydroxyl, β-coumarinyl substituted aldehyde. Although the subsequent β-elimination step was rate limiting under chemical or enzymatic catalysis, we detected a TK activity as low as 0.7 mIU. To improve the fluorescence signal release, kinetic and product distribution analyses of this reaction were performed by LC/UV/MS coupling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 145, Issue 4, 15 February 2010, Pages 359–366
نویسندگان
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