کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
24571 | 43527 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An improved and cost-effective cDNA-AFLP method to investigate transcription-derived products when high throughput sequencing is not available
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although the cost of high throughput sequencing is decreasing, the cost is still often too high for individual projects targeted at, e.g., genome-wide transcription profiling in non-model organisms. Then, a low-cost alternative is cDNA-AFLP, which we have now considerably modified in order to develop a faster and simpler method to identify and analyze genes involved in specific, possibly adaptive characteristics. Particularly, we wanted to exclude repetitive PCR amplifications, extensive cloning and the presence of overlapping transcripts, which all lower the efficiency of the method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 145, Issue 1, 1 January 2010, Pages 43–46
Journal: Journal of Biotechnology - Volume 145, Issue 1, 1 January 2010, Pages 43–46
نویسندگان
Helena Korpelainen, Kirsi Kostamo,