کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2466799 | 1555375 | 2013 | 9 صفحه PDF | دانلود رایگان |
Campylobacter jejuni is a pathogen of the gastrointestinal tract of humans, but colonizes chickens for prolonged periods without causing disease. It is unclear what host and bacterial mechanisms maintain a non-inflammatory state in chickens. The present work was undertaken to characterize cytokine responses of chickens to purified lipooligosaccharide (LOS) of C. jejuni HS:10. Chickens were injected with purified LOS, and expression of interleukin (IL)-1β (pro-inflammatory cytokine), IL-8 (pro-inflammatory chemokine), interferon (IFN)γ (Th1-like cytokine), IL-10 (immune regulatory/anti-inflammatory cytokine) and IL-13 (Th2-like cytokine) was evaluated in spleen using quantitative RT-PCR, up to 24 h post-injection. In an in vitro study, splenocytes were incubated with LOS, and cytokine expression followed up to 18 h. Chickens injected with LOS had increased expression of IL-1β up to 24 h later. Expression of IL-8 was significantly increased at 2 h but then declined below baseline. Expression of IFNγ and IL-10 was increased significantly at 2 h, but declined thereafter. Splenocytes incubated with LOS had increased expression of IL-1β and IL-8 up to 18 h of incubation. Expression of IFNγ was increased at 6 and 18 h, IL-10 was increased at 2 h, but expression of IL-13 did not differ significantly up to 18 h. It is concluded that LOS of C. jejuni can induce expression of pro-inflammatory IL-1β and IL-8, as well as IFNγ and IL-10 in chickens. More extensive studies with more prolonged exposure to LOS are needed to further clarify the interaction between C. jejuni and the chicken host.
Journal: Veterinary Microbiology - Volume 164, Issues 1–2, 31 May 2013, Pages 122–130